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KMID : 0603520020070030161
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Journal of Korean Association of Cancer Prevention
2002 Volume.7 No. 3 p.161 ~ p.167
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Impaired Growth of Telomerase-Deficient Cells by Telomere Dysfunction
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Ju Yeun-Jin
Park Jeong-Eun
Juhn Kyoung-Mi
Kim Chang-Min
Kim Sang-Hoon
Park Won-Bong
Lee Kee-Ho
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Abstract
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Gradual loss of telomere ends limits the growth capacity of primary cells whereas telomerase reactivation facilitates telomere maintenance and immortal cell growth in over 85% of human cancers. While telomerase activity is not detectable in most human somatic cells, inhibition of telomerase activity is thought to be a potential target for anticancer therapies. Here we show that telomerase inhibition has a profound effect on cell growth in mouse embryonic fibroblasts (MEFs) derived from telomerase-deficient mice. Telomere dysfunction exemplified by the formation of chromosomal end-to-end fusions was found to be an important factor of the growth impairment in telomerase-deficient cells. Multi-chromosomal fusion after exposure to doxorubicin is a typical characteristic of chromosomal abnormality. These findings indicate that chromosomal end-to-end fusion is a critical factor for the impairment of cell growth in telomerase-deficient cells and multi-chromosomal fusion might be one mechanistic basis of doxorubicin in conjunction with telomere function.
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KEYWORD
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Telomerase, Telomere, Chromosomal end to fusion, Cell growth
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